I've gone over the differences in Server and Web Publishing Rules and how they behave depending on whether there is a ROUTE or NAT Network Rule connecting the source and destination ISA Firewall Networks. However, when something is worth communicating, its worth communicating over and over and by different authors. This helps get the word out and keeps the issue alive so that we don't forget how things work and don't make mistakes in the future.
Philipp Sand, an ISA Firewall support specialist for Microsoft did a great job describing how publishing rules work in ROUTE and NAT relationships. He also provides some nice tips on how to use the command line tool fwengmon to troubleshoot ISA Firewall configuration issues.
Check out Philipp's article at:
Thomas W Shinder, M.D.
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MVP — Microsoft Firewalls (ISA)